The NPDI and Signum Dermalogix are collaborating to characterize components of this Natural Product collection for possible dermatological uses. Dermalogix develops cosmetic and drug candidates for skin care and have in-house screening capabilities to assess the activity and safety of potential cosmetic ingredients. Utilizing a cell-free radical scavenging assay, well-characterized natural extracts from the NPDI library are tested to identify those with the most potent radical scavenging capacities (antioxidant properties). Hits are then screened for anti-inflammatory in a dermal cell line. This work describes our recent screening results in profiling a pilot set of diverse samples and demonstrate the cosmetic product opportunities within this collection.
- 12 antioxidant extracts were identified from a total of 1,999 samples (0.6% hit rate) with potency rivaling ferulic acid.
- 11 of the hit extracts had never been examined for an active component.
- Most of the active extracts were from plant sources.
- Several extracts demonstrated broad anti-inflammatory activity in keratinocytes regardless of stimuli (UVB or TPA).
- Two extracts had beneficial effects on Fibroblasts.
- Extract 11 was unique in demonstrating anti-inflammatory activity in both keratinocytes and dermal fibroblasts.
- Screening of a small portion (2%) of the NPDI collection demonstrates its potential for cosmetic uses
The Natural Products Discovery Institute (NPDI) houses the former Merck U.S. natural product collection and makes it available to qualified researchers in both academic and industrial settings. This proven druggable collection amounts to over 100,000 extract samples (plants, fungi, and actinomycetes sourced), which are backed up with reserve materials and producing cultures, as well as extensive expertise in chemistry and microbiology garnered from decades of experience in a pharmaceutical setting. Early experience with the screening of the NPDI collection for pharmaceutical uses has yielded virgin hit extracts in 80% of cases (i.e. they had not been previously identified as biologically active nor explored for a bioactive component).